Afkham's remarks came after media reports said on Monday that Yemeni President Abdrabuh Mansur Hadi has accused Iran of supporting certain extremist groups in the Arab country.
"These groundless accusations are occasionally raised, but the falseness of such claims quite clear to everyone," Afkham told reporters at her weekly press briefing in Tehran on Tuesday.
She noted that such remarks are made despite the ongoing meddling and moves by some regional countries in Yemen contrary to the country’s interests and security.
The Iranian spokesperson pointed to relations between the two countries, and said, "The Islamic Republic has always supported unity, stability and integrity in Yemen."
“We believe that the issues in Yemen can only be resolved through dialog and a political process in line with meeting the legitimate demands of the country’s people and with the cooperation and participation of all the country’s political groups, parties and currents without foreign interference,” she added.
In relevant remarks last year, a senior Iranian parliamentarian rejected enemies' allegations about Iran's espionage operations in some certain countries, particularly in Yemen, underlining that the country follows up transparent policies.
"Iran does not need to carry out espionage operations in any country in the region because the Islamic Republic pursues its policies clearly," Rapporteur of the Iranian Parliament's National Security and Foreign Policy Commission Seyed Hossein Naqavi Hosseini said.
Unlike the era of Cold War, when espionage was conducted by spies, nowadays there is no need for the physical presence of people in other countries due to technological advances in acquiring information Naqavi Hosseini added.